Middlebrook 7H9 Agar Base

201130510500

MSDS
Technical Data

APPLICATION

Clinical Microbiology

Middlebrook 7H9 Agar Base is used for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis.

Composition*

Ingredients 
g/L
Ammonium Sulphate 
0.5
Sodium Glutamate 
0.5
Sodium Citrate 
0.1
Pyridoxine 
0.001
Biotin 
0.0005
Disodium Phosphate 
2.5
Monopotassium Phosphate
1.0
Ferric Ammonium Citrate 
0.04
Magnesium Sulphate 
0.05
Calcium Chloride 
0.0005
Zinc Sulphate 
0.001
Copper Sulphate 
0.001
Malachite Green 
0.001
Agar 
15.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 6.6 ± 0.2

g/L: Suspend 9.85 g in 450 mL purified / distilled water. 1 mL glycerol may be added if desired.

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