MRS Broth, Modified (Lactobacillus Heteroferm screen Broth) is a medium recommended for the isolation and cultivation of Lactobacillus species from salad dressings.
Composition*
Ingredients
| g/L
|
Dextrose
| 20.0
|
Proteose Peptone
| 10.0
|
Yeast Extract
| 5.0
|
Sodium Acetate
| 5.0
|
2-Phenylethyl Alcohol
| 3.0
|
Ammonium Citrate
| 2.0
|
Dipotassium Phosphate
| 2.0
|
Magnesium Sulphate
| 0.1
|
Manganese Sulphate
| 0.05
|
Bromocresol Green
| 0.04
|
Cycloheximide
| 0.004
|
*Adjusted to suit performance parameters.
Final pH (at 25°C) : 4.3 ± 0.2
g/L: Suspend 47.20 g of the powder in 1000 mL purified / distilled water containing 1mL Polysorbate 80.
