M17 Broth

M17 Broth medium is used for cultivation of lactic Streptococci and plaque assay of lactic bacteriophages. 

Composition*

Ingredients	g/L
Peptic Digest of Animal Tissue	2.5
Casein Enzymic Hydrolysate	2.5
Papaic Digest of Soyabean Meal	5.0
Yeast Extract	2.5
Beef Extract	5.0
Lactose	5.0
Ascorbic Acid	0.5
Disodium - ß - glycerophosphate	19.0
Magnesium Sulphate	0.25

*Adjusted to suit performance parameters.
Final pH (at 25°C):  7.1 ± 0.1

g/L: Suspend 42.25 g of the powder in 1000 mL purified / distilled water.