Michrom^TM Chromogenic Coliform Agar ISO

A medium used for detection of Escherichia coli and coliforms in water as well as clinical samples in compliance with ISO specification ISO 9308-1:2014.

Composition* 

Ingredients	g/L
Tryptone #	1.0
Yeast Extract	2.0
Sodium Chloride	5.0
Sodium Dihydrogen Phosphate, Dihydrate	2.2
Disodium Hydrogen Phosphate	2.7
Sodium Pyruvate	1.0
Sorbitol	1.0
Tryptophan	1.0
Tergitol-7	0.15
6-Chloro-3-Indoxyl β-D-galactopyranoside	0.2
5-Bromo-4-Chloro-3-Indoxyl- β-D-glucuronic acid Cyclohexamine ammonium salt, monohydrate	0.1
IPTG (Isopropyl- β-D-thiogalactopyranoside)	0.1
Agar	15.0

*Adjusted to suit performance parameters.
Final pH (at 25°C):  6.8 ± 0.2

g/L: Suspend 30.92 g (the equivalent weight of the dehydrated medium per litre) of the powder in 1000 mL purified / distilled water.