Middlebrook 7H10 Agar Base

201130520500

MSDS
Technical Data

APPLICATION

Clinical Microbiology

Middlebrook 7H10 Agar Base is recommended for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis.

Composition*

Ingredients 
g/L
Ammonium Sulphate 
0.5
L-Glutamic Acid 
0.5
Monopotassium Phosphate
1.5
Disodium Phosphate
1.5
Sodium Citrate 
0.4
Ferric Ammonium Citrate
0.04
Magnesium Sulphate 
0.025
Calcium Chloride 
0.0005
Zinc Sulphate 
0.001
Copper Sulphate 
0.001
Pyridoxine Hydrochloride
0.001
Biotin 
0.0005
Malachite Green 
0.00025
Agar 
15.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 6.6 ± 0.2

g/L: Suspend 9.73 g of the powder in 450 mL purified / distilled water containing 2.5 mL glycerol.

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