Middlebrook 7H11 Agar

201130530500

Middlebrook 7H11 Agar is recommended for isolation, cultivation and sensitivity testing of Mycobacteria.

Composition*

Ingredients 
g/L
Casein Enzymic Hydrolysate
1.0
Ammonium Sulphate  0.5
Monopotassium Phosphate
1.5
Disodium Phosphate 
1.5
Sodium Citrate 
0.4
Magnesium Sulphate 
0.05
L-Glutamic Acid 
0.5
Ferric Ammonium Citrate
0.04
Pyridoxine 
0.001
Biotin
0.0005
Malachite Green 
0.001
Agar 
15.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 6.6 ± 0.2

g/L: Suspend 10.25 g of the powder in 450 mL purified / distilled water containing 2.5 mL glycerol.

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