APPLICATION

Food

Clinical Microbiology

Culture Media for Bacteria & Fungi

Simmons Citrate Agar is used for the differentiation of Gram-negative bacteria on the basis of citrate utilization.

Composition*

Ingredients	g/L
Sodium Chloride	5.0
Sodium Citrate	2.0
Dipotassium Phosphate	1.0
Ammonium Dihydrogen Phosphate	1.0
Magnesium Sulphate	0.2
Bromothymol Blue	0.08
Agar	15.0

*Adjusted to suit performance parameters.
Final pH (at 25°C): 6.8 ± 0.2

g/L: Suspend 24.28 g of the powder in 1000 mL purified / distilled water and mix thoroughly.

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Acetobacter Broth (Mannitol)
Actinomycetes Isolation Agar
Aleksandrow Agar
Aleksandrow Broth
Algae Culture Agar
Alicyclobacillus Agar
Alkaline Peptone Water
Alternative Thioglycollate Medium
Amies Transport Medium Without Charcoal
Anaerobic Agar
Andrade Peptone Water
Andrade Peptone Water BIS
Antibiotic Assay Agar D
Antibiotic Assay Medium A (No.1) (Seed Agar)
Antibiotic Assay Medium B BP
Antibiotic Assay Medium D
Antibiotic Assay Medium E (No.5)
Antibiotic Assay Medium F
Antibiotic Assay Medium H
Antibiotic Assay Medium No.10
(Polymyxin Seed Agar)
Antibiotic Assay Medium No.11
(Neomycin, Erythromycin Assay Agar)
Antibiotic Assay Medium No.19
Antibiotic Assay Medium No.3 (Assay Broth)
Antibiotic Assay Medium No.32
Antibiotic Assay Medium No.35
Antibiotic Assay Medium No.4
(Yeast Beef Agar)
Antibiotic Assay Medium No.8
Antibiotic Assay Medium No.9
Antifungal Assay Agar
Antimycotic Sensitivity Test Agar
Arginine-Glucose Yeast Extract Agar
Artificial Sea Water
Ashby’s Mannitol Agar
Ashby’s Mannitol Broth
Asparagine Proline Broth
Aspergillus Differentiation Medium Base
Azide Dextrose Broth
Azotobacter Agar (Mannitol)
Azotobacter Broth (Dextrose)
Azotobacter Broth (Mannitol)
B.Q. Vaccine Medium
B12 Assay Agar
(using E. coli Mutant Culture)
B12 Assay Medium (Lactobacillus leichmannii Culture)
B12 Maintenance Medium
(for E. coli mutant)
Bacillus Cereus Agar Base
Baird Parker Agar Base
Baird Parker Agar Base (Agar Medium O) EP
Baird Parker Agar Base
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Baird Parker Agar Base BIS
Baird Parker Agar Medium USP
Bifidobacterium Agar
Bile Broth
Bile Esculin Agar
Bile Esculin Azide Agar
Bile Salt Agar
Bile Salt Agar BIS
Bismuth Glucose Glycine Yeast Agar (BiGGY)
Bismuth Sulphite Agar
Bismuth Sulphite Agar Medium USP
Blood Agar Base (Infusion Agar)
Blood Agar Base No. 2
Brain Heart Infusion Agar
Brain Heart Infusion Broth
Brain Heart Infusion with PABA and Agar
Brewer Thioglycollate Medium
Brilliant Green Agar, Medium USP
Brilliant Green Agar, Modified
Brilliant Green Bile Agar
Brilliant Green Bile Broth 2%
Brilliant Green, Phenol Red, Lactose Monohydrate, Sucrose Agar (Agar Medium L) BP
Brilliant Green, Phenol Red, Lactose Monohydrate, Sucrose Agar (Agar Medium L) EP
Brucella Agar Base
Brucella Broth Base
Buffered Charcoal Yeast Extract Agar
Buffered Peptone Water
Buffered Peptone Water BIS
Buffered Peptone Water ISO
Buffered Peptone Water with NaCl
Buffered Sodium Chloride-Peptone Solution pH 7.0 (Harmonized)
Burkhloderia Cepacia Selective Agar Base USP
Burkholderia Cepacia Agar Base
C.L.E.D. Agar with Andrade Indicator
C.L.E.D. Agar with Bromothymol Blue
Campylobacter Agar Base
Carbohydrate Consumption Broth Base
Cary-Blair Medium Base
(Transport Medium w/o Charcoal)
Cetrimide Agar (Harmonized)
Cetrimide Agar Base
Chloramphenicol Yeast Glucose Agar
Clausen Medium
Clostridial Agar
Clostridium Butyricum Agar
Columbia Agar (Harmonized)
Columbia Blood Agar Base
Columbia Blood Agar Base E
Cooked Meat Medium
(Robertson Cooked Meat Medium)
Cooked Meat Medium with Glucose,
Hemin and Vitamin K
Corn Meal Agar
Culture Medium for RWC
(Disinfectant Test Broth)
Czapek Dox Agar
Czapek Dox Broth
D-Mannitol, A. R. Sterile (Gamma-Irradiated)
Decarboxylase Agar Base
Decarboxylase Broth Base, Moeller
(Moeller Decarboxylase Broth Base)
Deoxycholate Citrate Agar
Deoxycholate Citrate Agar (Agar Medium J) EP
Deoxycholate Citrate Agar BIS
Deoxycholate Citrate Agar BP
Deoxycholate Lactose Agar
Dermatophyte Test Agar Base
Dey-Engley Neutralizing Agar
(D/E Agar Disinfectant Testing)
Dey-Engley Neutralizing Broth
(D/E Broth Disinfectant Testing)
Dichloran Glycerol Medium Base ISO
Dichloran Rose Bengal Chloramphenicol (DRBC) Agar
Differential Reinforced Clostridial Agar Base
Differential Reinforced Clostridial Broth
Differential Reinforced Clostridial Broth Base ISO
Diluting Fluid A
Diluting Fluid D
Diluting Fluid K
Dixon’s Agar (Twin Pack)
DNase Test Agar Base
E.C. Broth
Egg Yolk Agar Base
Elliker Broth
Ellners Broth
EMB Agar
EMB Agar, Levine
EMB Agar, Levine BIS
EMB Agar, Levine USP
EMB Broth
Endo Agar
Enterobacteria Enrichment Broth, Mossel (Harmonized)
Enterobacteria Enrichment Broth, Mossel
(Medium 5) IP
Enterococcus Confirmatory Agar
Ethyl Violet Azide Broth
Ethyl Violet Azide Dextrose Agar
Fluid Casein Digest Soya Lecithin
Polysorbate 20 Medium (Twin Pack) USP
Fluid Lactose Medium
Fluid Lactose Medium USP
Fluid Selenite Cystine Medium (Twin Pack)
Fluid Selenite Cystine Medium (Twin Pack) ISO
Fluid Selenite Cystine Medium (Twin Pack) USP
Fluid Tetrathionate Medium USP
Fluid Thioglycollate Medium
Fluid Thioglycollate Medium BP
Fluid Thioglycollate Medium EP
Fluid Thioglycollate Medium IP
Fluid Thioglycollate Medium USP
Fluid Thioglycollate Medium VEG
Fraser Broth Base
Fraser Broth Base ISO
Fraser Secondary Enrichment Broth Base
G C Agar Base
G C Broth
Gardnerella Selective Agar
Gelatin Agar
Glucose Agar
Glucose Broth
Glucose Citrate Broth
Glucose Salt Teepol Broth (Twin Pack)
Glucose Yeast Extract Agar
GN Broth IP (Medium 11)
Hartley's Digest Broth
Hektoen Enteric Agar
Heterotrophic Plate Count Agar
Hugh Leifson Medium
Hugh Leifson Medium BIS
Indole Nitrate Medium
(Tryptone Nitrate Medium)
Iron Sulphite Agar
Iron Sulphite Agar, Modified
Isolation Medium for Iron Bacteria
Jensen's Broth
Jensen's Medium
K Agar
KF Streptococcus Agar Base
KF Streptococcus Agar Base w/BCP
King's Medium A Base
King's Medium B Base
King's Medium B Broth
Kligler Iron Agar
Kligler Iron Agar ISO
Lactobacilli MRS Broth W/O Glucose
Lactobacillus Bulgaricus Agar Base
Lactobacillus MRS Agar
Lactobacillus MRS Broth
Lactose Broth
Lactose Broth BIS
Lactose Monohydrate Broth
(Broth Medium D) EP
Lactose Monohydrate Broth
(Broth Medium D) BP
Lactose Monohydrate Sulphite Medium
(Medium R)
Lactose Monohydrate, Sterile
(Gamma-Irradiated)
Lactose Sulphite Broth Base
Lactose TTC Agar with Tergitol
Lauryl Tryptose Broth
(Lauryl Sulphate Broth)
Lauryl Tryptose Mannitol Broth
W / Tryptophan
Legionella Agar Base
Leptospira Medium Base EMJH
Letheen Agar
Letheen Agar, Modified
Letheen Broth (Twin Pack)
Letheen Broth, Modified (Twin Pack)
Listeria Enrichment Broth
Listeria Identification Agar Base (PALCAM)
Listeria Identification Agar Base (PALCAM) VEG
Listeria Identification Broth Base (PALCAM)
Listeria Oxford Medium Base
Listeria Selective Agar
Loeffler Medium Base
Lowenstein Jensen Medium Base
Luria Agar
Luria Bertani Agar, Miller
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Luria Broth
Lysine Decarboxylase Broth
Lysine Iron Agar
M 17 Agar
M-Bismuth Sulphite Broth
M-Brilliant Green Broth
M-CP Agar
M-Endo Agar LES
M-Endo Broth
M-FC Agar Base
M-FC Agar Modified For Klebsiella
M-MacConkey Broth
M-Nutrient Broth
M17 Broth
M7HrFC Agar
MacConkey Agar (Harmonized)
MacConkey Agar Base
MacConkey Agar BIS
MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts
MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts (Medium 8) IP
MacConkey Agar without Crystal Violet and with 0.15% Bile Salts
MacConkey Agar without Crystal Violet, NaCl and with 0.15% Bile Salts
MacConkey Agar without Crystal Violet, NaCl and with 0.5% Bile Salts
MacConkey Agar without Crystal Violet, NaCl and with 0.5% Sodium Taurocholate
MacConkey Broth (Harmonized)
MacConkey Broth Double Strength with Neutral Red
MacConkey Broth Double Strength with Neutral Red BIS
MacConkey Broth Purple with BCP
MacConkey Broth Purple with BCP (Medium 7) IP
MacConkey Broth with Neutral Red
MacConkey Broth with Neutral Red BIS
MacConkey Sorbitol Agar Base
Malachite Green Broth
Malonate Broth
Malonate Broth Ewing Modified
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Malt Extract Yeast Extract Glucose Agar
(MY 40G Agar)
Mannitol Motility Test Medium
Mannitol Salt Agar
Mannitol Salt Agar (Harmonized)
Mannitol Salt Agar (Medium 14) IP
Marine Agar 2216 (Zobell Marine Agar)
Marine Broth 2216 (Zobell Marine Broth)
Maximum Recovery Diluent
Michrom^TM Candida Differential Agar
Michrom^TM Chromogenic Coliform Agar ISO
Michrom^TM Chromogenic Coliform Agar with SLS
Michrom^TM Coliform Agar (Chromogenic Coliform Agar)
Michrom^TM E. Coli Agar
Michrom^TM Enterococcus Agar
Michrom^TM Hydrocheck
Michrom^TM UTI Agar
Michrom^TM Vibrio Agar
Middlebrook 7H10 Agar Base
Middlebrook 7H11 Agar
Middlebrook 7H9 Agar Base
Milk Agar
Milk Agar with Cetrimide (Twin Pack)
Milk Medium with Reducing Agent
Mitis Salivarius Veg Agar Base
MIU Medium Base
Modified Listeria Oxford Agar Base
Modified Oxford Agar
Modified Rappaport Vassiliadis Medium
Moeller Decarboxylase Broth with Arginine
Moeller Decarboxylase Broth with Lysine
Moeller Decarboxylase Broth with Ornithine
Motility Test Medium
Motility Test Medium (Edwards and Ewing) BIS
MR -VP Medium (Glucose Phosphate Broth or Buffered Glucose Broth) BIS
MR -VP Medium
(Glucose Phosphate Broth or
Buffered Glucose Broth)
MRS Broth (De Man, Rogosa and Sharpe Broth)
MRS Broth, Modified (Lactobacillus Heteroferm screen Broth)
Mueller Hinton Agar
Mueller Hinton Agar No. 2
Mueller Hinton Agar with 2% Glucose and
Methylene Blue
Mueller Hinton Broth
Mueller Kauffmann Tetrathionate Broth Base (Novobiocin Broth)
MUG EC Broth
MYP Agar
(Phenol Red Egg Yolk Polymyxin Agar Base)
Neutralizing Fluid EP
Nitrate Broth BIS
Nutrient Agar
Nutrient Agar BIS
Nutrient Agar No. 2
Nutrient Agar VEG
Nutrient Agar with 1% Peptone
Nutrient Agar with Skim Milk
Nutrient Broth
Nutrient Broth No. 2
Nutrient Broth VEG
Nutrient Broth with 1% Peptone
Nutrient Gelatin (Twin Pack)
O F Basal Medium
OFPBL Agar Base (Oxidation Fermentation Polymyxin Bacitracin Lactose Agar Base)
Orange Serum Agar
Ornithine Decarboxylase Broth
Peptone Salt Broth
Peptone Water
Peptone Water BIS
Perfringens Agar Base
Pfizer Selective Enterococcus Agar
Phenol Red Agar Base
Phenol Red Broth Base
Phenol Red Dextrose Agar
Phenol Red Dextrose Broth
Phenol Red Lactose Agar
Phenol Red Lactose Broth
Phenol Red Maltose Broth
Phenol Red Mannitol Broth
Phenol Red Sorbitol Broth
Phenol Red Sucrose Agar
Phenol Red Sucrose Broth
Phenylalanine Agar
Phosphate Buffered Saline, pH 7.2
Pikovskaya’s Agar
Pikovskaya’s Broth
Plate Count Agar (Standard Methods Agar)
Plate Count Agar
(Standard Methods Agar) BIS
PNY Medium
Potato Dextrose Agar
Potato Dextrose Agar (Harmonized)
Potato Dextrose Agar for Trichoderma
Potato Dextrose Broth
Potato Dextrose Broth for Trichoderma
Potato Infusion Agar
Pseudomonas Agar Base
Pseudomonas Agar Medium for Detection of Fluorescein
Pseudomonas Agar Medium for Detection of Pyocyanin
Pseudomonas Isolation Agar Base
Purple Agar Base
R-2A Agar
R-2A Agar (Agar Medium S) BP
R-2A Agar (Agar Medium S) EP
R-2A Broth
Rapid Chromogenic Coliform Broth
Rappaport Vassiliadis Salmonella Enrichment Broth IP (Medium 9)
Rappaport Vassiliadis Salmonella Enrichment Broth
(Harmonized)
Reinforced Clostridial Agar
Reinforced Medium for Clostridia (Harmonized)
Reinforced Medium for Clostridia (Medium 15) IP
Rhizobium Medium
Rogosa S L Agar
Rose Bengal Agar Base
Rose Bengal Chloramphenicol Agar
Sabouraud Chloramphenicol Agar
Sabouraud Cycloheximide Chloramphenicol Agar
Sabouraud Dextrose Agar
Sabouraud Dextrose Agar (Harmonized)
Sabouraud Dextrose Agar with Antibiotic(S)
(Medium 4) IP
Sabouraud Dextrose Agar, Modified
(Dextrose Agar Base, Emmons)
Sabouraud Dextrose Agar
with Soya Lecithin and Polysorbate 80
Sabouraud Dextrose Broth
Sabouraud Dextrose Broth (Harmonized)
Sabouraud Glucose Agar
Sabouraud Glucose Broth
Sabouraud Maltose Agar
Salmonella Selective Enrichment Broth
Salt Meat Broth
Selenite Cystine Broth Base without Biselenite
Selenite F Broth (Twin Pack)
Shigella Broth Base
SIM Medium
Simmons Citrate Agar BIS
Skim Milk Agar
Soyabean Casein Digest Agar (Harmonized)
Soyabean Casein Digest Agar (Tryptone Soya Agar)
Soyabean Casein Digest Agar
(Casein Soyabean Digest Agar) IP (Medium 2)
Soyabean Casein Digest Medium
(Tryptone Soya Broth)
Soyabean Casein Digest Medium
with Lecithin and Polysorbate 80
Soyabean Casein Digest Medium VEG
Soyabean Casein Digest Medium, Sterile Powder
Soyabean Casein Digest Medium
(Tryptone Soya Broth) (Harmonized)
SS (Salmonella Shigella Agar) Agar
Standard Nutrient Agar
Standard Nutrient Broth (H. S. Vaccine Medium)
Starch Agar
Starch Casein Agar
Streptococcus Thermophilus Isolation Agar
Stuart Transport Medium
TAT Broth Base
Taurocholate Broth
TCBS Agar
TCBS Agar - Selective Media
Tellurite Blood Agar Base
Tellurite Glycine Agar Base
Tergitol-7 Agar Base
Tetrathionate Brilliant Green Bile Broth
Tetrathionate Brilliant Green Bile Broth IP
Tetrathionate Broth Base, Hajna
Thiobacillus Agar
Thiobacillus Broth
Thioglycollate Agar
Thioglycollate Broth
Todd Hewitt Broth
Toluidine Blue DNA Agar
Tributyrin Agar w/o Tributyrin
Triple Sugar Iron Agar
Triple Sugar Iron Agar BIS
Tryptone Bile Agar
Tryptone Glucose Beef Extract Agar
(TGB Agar)
Tryptone Glucose Yeast Extract Agar
Tryptone Salt Agar with 1% NaCl
Tryptone Salt Agar with 3% NaCl
Tryptone Salt Broth
Tryptone Soya Agar with Added NaCl
Tryptone Soya Agar with Lecithin and Polysorbate 80 (Soyabean Casein Digest Agar
with Lecithin and Polysorbate 80)
Tryptone Soya Broth with 4% Polysorbate 20 and 0.5% Lecithin (Twin Pack)
Tryptone Soya Broth with Phenol Red
Tryptone Soya Yeast Extract Agar ISO
Tryptone Water (Tryptone Broth)
Tryptone Water without Sodium Chloride
Tryptophan Broth
Tryptose Blood Agar Base
Tryptose Phosphate Broth
TSB CAP 4 with Tween 80
Urea Agar Base, Christensen
Urea Broth Base
Violet Red Bile Agar BIS
Violet Red Bile Agar with MUG
Violet Red Bile Agar
(Crystal Violet Neutral Red Bile Lactose Agar)
Violet Red Bile Glucose Agar (Harmonized)
Violet Red Bile Glucose Agar IP
(Medium 6)
Violet Red Bile Glucose Agar w/ Lactose
(Crystal Violet, Neutral Red, Bile Agar
with Glucose) (Agar Medium F) BP
Violet Red Bile Glucose Agar without Lactose
Violet Red Bile Glucose Agar without Lactose ISO
Vogel Johnson Agar Base w/o Tellurite
Willis and Hobbs Medium Base
Wilson Blair Agar Base
Wilson Blair Agar w/ BG
Xylose Lysine Deoxycholate Agar
Xylose Lysine Deoxycholate Agar (Harmonized)
Xylose Lysine Deoxycholate Agar (Medium 12) IP
Xylose Lysine Deoxycholate Agar, Modified ISO
Yeast Extract Chloramphenicol Dextrose Agar
Yeast Lactose Agar
Yeast Malt Agar (YM Agar)
Yeast Mannitol Agar with Congo Red
Yeast Mannitol Broth
Yeast Nitrogen Base
Yeast Nitrogen Base Agar (Twin Pack)
YEM Agar
YEM Broth
YPD (YEPD Growth Agar) Agar
YPD Broth (YEPD Broth)
YSG Agar
YSG Broth