TCBS Agar - Selective Media

201200020500

This medium is recommended for the selective isolation of Vibrio cholerae and other enteropathogenic Vibrios.

Composition* 

Ingredients
g/L
Peptone, Special
10.0
Yeast Extract
5.0
Sodium Citrate
10.0
Sodium Thiosulphate
10.0
Sodium Cholate
3.0
Oxgall
5.0
Sucrose
20.0
Sodium Chloride
10.0
Ferric Citrate
1.0
Bromothymol Blue
0.04
Thymol Blue
0.04
Agar
15.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 8.8 ± 0.2

g/L: Suspend 89.08 g of the powder in 1000 mL purified / distilled water.

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