Oxidase Reagent is used to detect the presence of an enzyme cytochrome oxidase found in some bacteria.
The Gordon-McLeod Reagent serves as an alternate substrate for the cytochrome oxidase reaction. In the reduced state N, N, N', N-tetramethyl-p-phenylenediamine dihydrochloride is colourless but when oxidized it becomes dark purple due to the formation of indophenol blue indicating a positive reaction.
Reagents/contents :
The Gordon-McLeod Reagent comprises of:
1. 0.5% N, N, N', N-tetramethyl-p-phenylenediamine dihydrochloride.
2. Distilled water
Appearance: Amber coloured with an alcoholic odour.
