TDA Reagent is used for phenylalanine deamination reaction in differentiating Proteus from other members of Enterobacteriaceae.
This test detects the ability of an organism to oxidatively deaminate phenylalanine with production of phenylpyruvic acid. On addition of TDA reagent to the medium, after incubation, phenyl pyruvic acid produced reacts with ferric salts in the reagent to give a green colour appearance indicating a positive reaction. No change in colour indicates negative reaction.
Reagents/contents :
The TDA Reagent (Ferric Chloride Reagent) comprises of:
1. 10% Ferric Chloride.
Appearance: Light yellow coloured clear solution.
