Test Kit for MR-VP is used for detection of acid and acetoin production and for differentiation of Enterobacteriaceae.
In general, different bacteria convert dextrose and glucose to pyruvate using different metabolic pathways. Some of these pathways produce unstable acidic products, which quickly convert to neutral compounds. Some organisms use the butylene glycol pathway, which produces neutral end products including acetoin and/or 2,3-butanedioI. Other organisms use the mixed acid pathway, which produces stable acidic end products such as tactic, formic and acetic acid. A medium like MR-VP Broth is used to determine the pathway a given organism uses to ferment glucose; to this end reagents like Methyl Red Indicator or Barritt Reagent A and Barritt Reagent B are added along with Creatine to detect end products or intermediates produced by a given fermentation pathway.
Reagent:
1. 5 vials containing 1 mL medium each for MR test.
2. 5 vials containing 0.2 mL medium each for VP test.
