MacConkey Agar without Crystal Violet, NaCl and with 0.5% Bile Salts is used for the cultivation and differentiation of enteric bacteria, restricting swarming of Proteus species as well as potentially pathogenic Gram-positive organisms.
Composition*
Ingredients
| g/L
|
Peptic Digest of Animal Tissue
| 20.0
|
Bile Salts
| 5.0
|
Lactose
| 10.0
|
Neutral Red
| 0.075
|
Agar
| 12.0
|
*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.4 ± 0.2
g/L: Suspend 47.07 g of the powder in 1000 mL purified / distilled water and mix thoroughly.