Triple Sugar Iron Agar

201200170100 / 201200170500

Triple Sugar Iron Agar is used for the identification of Gram-negative enteric bacilli on the basis of dextrose, lactose and sucrose fermentation and hydrogen sulphide production.

Composition*

Ingredients
g/L
Lactose
10.0
Sucrose
10.0
Peptone
10.0
Tryptone
10.0
Sodium Chloride
5.0
Yeast Extract
3.0
Beef Extract
3.0
Dextrose
1.0
Sodium Thiosulphate
0.3
Ferrous Sulphate
0.2
Phenol Red
0.024
Agar
12.0
*Adjusted to suit performance parameters.

  Final pH (at 25°C): 7.4 ± 0.2

g/L: Suspend 64.52 g of powder in 1000 mL purified / distilled water.

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