MRS Broth (De Man, Rogosa and Sharpe Broth)

201131560500

A medium used for the isolation and cultivation of Lactobacillus species from clinical specimens, foods and dairy products.

Composition*

Ingredientsg/L
Glucose18.5
Pancreatic Digest of Gelatin10.0
Beef Extract8.0
Yeast Extract4.0
Sodium Acetate3.0
Potassium Hydrogen Phosphate2.0
Ammonium Citrate2.0
Polysorbate 801.0
Magnesium Sulphate. 7 H2O0.2
Manganese Sulphate. 4 H2O0.05

*Adjusted to suit performance parameters.
 Final pH (at 25°C): 6.2 ± 0.2

g/L: Suspend 48.75 g of the powder in 1000 mL purified / distilled water.

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