Rappaport Vassiliadis Salmonella Enrichment Broth is a selective enrichment medium for Salmonella species from pharmaceutical products in accordance with microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP.
Rappaport et al., formulated an enrichment medium for Salmonella that was modified by Vassiliadis et al., Rappaport Vassiliadis Salmonella Enrichment Broth is a selective enrichment for Salmonella species. This medium is selective for Salmonella species because they are typically resistant to malachite green, high osmotic pressure and low pH. S. typhi and S. choleraesuis are sensitive to malachite green and may be inhibited.
Composition*
| Ingredients | g/L |
| Soya Peptone | 4.5 |
| Magnesium Chloride Hexahydrate | 29.0 |
| Sodium Chloride | 8.0 |
| Dipotassium Phosphate | 0.4 |
| Potassium Dihydrogen Phosphate | 0.6 |
| Malachite Green | 0.036 |
*Adjusted to suit performance parameters.
Final pH (at 25°C): 5.2 ± 0.2
