Sabouraud Dextrose Agar with 1% Glycerol Plate is used for cultivation of Yeasts, Moulds and Aciduric microorganisms.
Sabouraud Dextrose Agar Plate was formulated by Sabouraud in 1892 for culturing dermatophytes, later Carliers modified the formulation and same is used for the cultivation of fungi (yeasts, moulds), particularly useful for the fungi associated with skin infections.
The acidic pH of this medium inhibits the growth of bacteria but permits the growth of yeasts and most filamentous fungi. This medium is also employed to determine mycological evaluation of food, contamination in cosmetics and clinically to aid in the diagnosis of yeast and fungal infections.
Composition*
Ingredients
| g/L
|
Pancreatic Digest of Casein
| 5.0
|
Peptic Digest of Animal Tissue
| 5.0
|
Dextrose
| 40.0
|
Glycerol
| 1.0
|
Agar
| 15.0
|
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 5.6 ± 0.2
