Violet Red Bile Glucose Agar with Lactose (Crystal Violet, Neutral Red, Bile Agar with Glucose) (Agar Medium F) is recommended for detection and enumeration of Enterobacteriaceae from pharmaceutical products in accordance with the microbial limit testing by harmonized methodology of BP.
Composition*
Ingredients
| g/L
|
Pancreatic Digest of Gelatin
| 7.0
|
Yeast Extract
| 3.0
|
Sodium Chloride
| 5.0
|
Bile Salts
| 1.5
|
Glucose Monohydrate
| 10.0
|
Lactose Monohydrate
| 10.0
|
Neutral Red
| 0.03
|
Crystal Violet
| 0.002
|
Agar
| 15.0
|
*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.4 ± 0.2
g/L: Suspend 51.53 g of the powder in 1000 mL purified / distilled water.
