WBC Diluting Fluid

Diluting Fluid used for WBC count.

WBC diluting fluid is used for performing the WBC (Leucocyte) count. Glacial acetic acid lyses the red cells. Gentian violet slightly stains the nuclei of the leucocytes. The blood specimen is diluted 1:20 in a WBC pipette with the diluting fluid and the cells are counted under low power of the microscope by using a counting chamber. The number of cells in undiluted blood is reported per cumm (µl) of whole blood.

Appearance: Purple coloured, clear solution.