Test Kit for Esculin Hydrolysis is used for detection of esculin hydrolysis.
Group D Streptococci and Enterococci are able to split esculin. The test is based on detection of esculin hydrolysis and is used for the identification of group D Streptococci. Esculin is hydrolyzed by group D Streptococci to form dextrose and esculetin. Esculetin reacts with the ferric ions contained within the medium, turning the medium dark brown to black. Thus, tolerance to the presence of bile and hydrolysis of esculin provide the means to presumptively identify group D Streptococci. This test can also be used for identification of Listeria, since all Listeria species hydrolyse esculin.
Reagent:
10 vials containing 1 mL medium each for esculin hydrolysis.
