Rapid two step cold AFB stain.
Novachrom® staining system is simple to perform and very much reproducible. It has a sensitivity comparable to
the traditional Ziehl Neelsen hot staining method and also as compared to Acid Fast Bacilli culture results. It is a
clinically proven, easy to use, time, labor, and cost saving. This simplicity of staining makes Novachrom® an ideal
screening tool for Mycobacterium tuberculosis.
Infection with Mycobacterium tuberculosis remains a major public health problem. The epidemic of Tuberculosis
and Multi Drug Resistant Tuberculosis reflects the failure of public health and social programs towards prompt
treatment of infected cases and screening of high-risk population. While culture, isolation, and sensitivity of
Mycobacterium tuberculosis from patient groups using standard methods remain the gold standard for
Mycobacterium tuberculosis detection and effective and swift treatment worldwide, Acid Fast Bacilli staining is the
first line microscopic procedure performed towards this goal.
Reagent / Contents:
a) Novachrom® AFB stain (A) - Carbol Fuchsin Strong (1%)
b) Novachrom® AFB stain (B) - Loeffler’s Methylene blue
Appearance:
Novachrom® Stain (B) – Dark pink coloured liquid
Novachrom® Stain (B) – Dark blue coloured liquid
