May Grunwald Stain is used for staining of blood, bone marrow smears and clinical cytological specimens.
In hematology polychromatic Romanowsky dyes are a standard for blood smears and bone marrow staining. Various sorts of Romanowsky dyes (Giemsa, May-Grunwald, Leishman, Wright, Jenner) contain different ratios of methylene bluing reagent used as the cation component (and the reagent-related thiazine dyes, such as azure B) and eosin Y as the anion component. Cation and anion components interaction creates a well-known Romanowsky effect that cannot be achieved if each component is being used individually. Purple color indicates the effect's presence. Staining intensity depends on the azure B content, as well as azure B to eosin Y ratio, while a few other factors affect the result of staining: working solution pH value and buffer solution, fixation method and dye exposure time. May-Grunwald solution is used for staining bone marrow and peripheral blood smear; for staining lymphocytes, monocytes, granulocytes (neutrophils, eosinophils and basophils), thrombocytes and erythrocytes. The May-Grunwald solution is used in cytology to stain cyto-diagnostic puncture aspirates, cells from diarrhea and secretion. One of the well-known methods that use the May-Grunwald solution is in combination with the Giemsa solution in the May-Grunwald Giemsa, or Pappenheim method.
Reagents / Contents:
| Eosin Y | 1.0 g |
| Methylene blue | 1.0 g |
| Methanol | 100 mL |
**Formula adjusted, standardized to suit performance parameters
Appearance: Dark Blue colour solution..
