Ready to use combi kit each of May Grunwald Stain and Giemsa Stain for staining of blood, bone marrow smears and clinical cytological specimens.
May-Grunwald solution is used for staining bone marrow and peripheral blood smear; for staining lymphocytes, monocytes, granulocytes (neutrophils, eosinophils and basophils), thrombocytes and erythrocytes. The May-Gruenwald solution is used in cytology to stain cyto-diagnostic puncture aspirates, cells from diarrhea and secretion. One of the well-known methods that use the May-Grunwald solution is in combination with the Giemsa solution in the May-Grunwald Giemsa, or Pappenheim method. Giemsa staining makes the effect of azure more prominent for staining all cellular components. The basic dyes carry net positive charges; consequently, they stain nuclei (because of the negative charges of phosphate groups of DNA and RNA molecules), granules of basophil granulocytes and RNA molecules of the cytoplasm of white blood cells. The eosin carries net negative charge and stains red blood cells and granules of eosinophil granulocytes.
Reagents / Contents:
| May Grunwald | |
| Eosin Y | 1.0 g |
| Methylene blue | 1.0 g |
| Methanol | 100 mL |
| Giemsa Stain | |
| Azur II eosin | 3.0 g |
| Azur II | 0.8 g |
| Glycerine | 125 mL |
| Methyl alcohol, absolute | 375 mL |
**Formula adjusted, standardized to suit performance parameters
Appearance:
May Grunwald: Dark blue colour solution.
Giemsa Stain: Dark blue colour solution.
