Papanicolaou EA-36 is used in cytoplasmic staining of gynaecological and non-gynaecological samples.
PAP stain is a polychromatic
staining method containing multiple dyes to differentially stain various
components of the cells which is used to differentiate cells in smear
preparations of various bodily secretions. PAP stain is a very reliable
technique. Papanicolaou Stains are used in conjunction with Hematoxylin nuclear
stains in the diagnosis of malignant cytological disease. As such it is used
for cervical cancer screening. Cancer cells may be found by the smear technique
in imprints of puncture biopsy material and in smears of cervical cells, vaginal
secretion, prostatic secretion, urine, gastric contents, bronchial aspirations,
cavity fluids and sputum. A diagnosis of malignancy made from stained smears
should be considered tentative and should be checked by tissue sections.
Reagents / Contents:
Papanicolaou EA – 36
Light green 45.0 g
Bismark brown 10.0 g
Eosin Y 45.0 g
Phosphotungstic acid 0.20 g
Lithium carbonate, saturated aqueous solution 1 drop
Appearance: Green with pinkish/red tinge
solution
Papanicolaou EA – 65
Eosin Y 0.23%
Bismarck brown 0.05%
Fast green FCF 0.01%
Phosphotungstic acid, 0.2%, in denatured alcohol
Appearance: Green with pinkish-orange tinge solution
Papanicolaou OG – 6
Orange G-6 Certified 0.3 g
Phosphotungstic acid 0.015 g
Denatured alcohol 100.0 mL
Appearance: Orange colored solution.
