Papanicolaou EA-65 is used as a counterstain with Hematoxylin Stains in Papanicolaou staining procedure.
PAP stain is a polychromatic staining method containing multiple dyes to differentially stain various components of the cells which is used to differentiate cells in smear preparations of various bodily secretions. PAP stain is a very reliable technique. Papanicolaou Stains are used in conjunction with Hematoxylin nuclear stains in the diagnosis of malignant cytological disease. As such it is used for cervical cancer screening. Cancer cells may be found by the smear technique in imprints of puncture biopsy material and in smears of cervical cells, vaginal secretion, prostatic secretion, urine, gastric contents, bronchial aspirations, cavity fluids and sputum. A diagnosis of malignancy made from stained smears should be considered tentative and should be checked by tissue sections.
Reagents / Contents:
Papanicolaou EA – 36
Light green 45.0 g
Bismark brown 10.0 g
Eosin Y 45.0 g
Phosphotungstic acid 0.20 g
Lithium carbonate, saturated aqueous solution 1 drop
Appearance: Green with pinkish/red tinge solution
Papanicolaou EA – 65
Eosin Y 0.23%
Bismarck brown 0.05%
Fast green FCF 0.01%
Phosphotungstic acid, 0.2%,
Appearance: Green with pinkish-orange tinge solution
Papanicolaou OG – 6
Orange G-6 Certified 0.3 g
Phosphotungstic acid 0.015 g
Denatured alcohol 100.0 mL
Appearance: Orange colored solution.
