Ultra-Fast Papanicolaou Staining kit.
The original staining procedure was developed by George N. Papanicolaou who modified his hormonal status stain in order to visualize cancer cells. The three main advantages of this staining procedure are: (1) Good definition of nuclear detail. (2) Cytoplasmic clarity and transparency. (3) Indication of cellular differentiation of squamous epithelium. It is a polychrome staining method which depends on degree of cell maturity and cellular metabolic activity.
ULTRA-PAP is a modification of the classical PAP Stains to give an Ultra-Fast Papanicolaou Stain with a formulation which reduces the time needed for staining along with a simplified procedure. This Ultra-Fast procedure gives clear nuclear and cytoplasmic staining.
Reagents / Contents :(for 250 Smears)
| ULTRA-PAP Nuclear Stain | 100 mL |
| ULTRA-PAP Cyto-Stain A | 55 mL |
| ULTRA-PAP Cyto-Stain B | 55 mL |
| Scotts Tap Water Buffer | 35 mL |
| Micro-Fix Fixative Spray | 2 x 50 mL |
| Dehydrant (IPA) | 3 x 100 mL |
| Xylene | 2 x 100 mL |
| D.P.X. Mounting Medium | 15 mL |
Appearance
Ultra-PAP Nuclear Stain : Maroon purplish solution
Ultra-PAP Cyto Stain A : Orange solution
Ultra-PAP Cyto Stain B : Dark greenish solution
Scott's Tap Water Buffer : Clear, colourless solution
MicroFix Fixative Spray : Clear, colourless solution
Dehydrant (IPA) : Clear colourless solution
Xylene : Clear, colourless solution
D.P.X. Mounting Medium : Clear, colourless thick solution
