Tryptone Soya Agar with Lecithin, Polysorbate 80, Histidine and Sodium Thiosulphate Plate is used for validation of cleanliness on surfaces of containers, equipment surfaces and water miscible cosmetics etc.
In 1948, Weber and Black reported lecithin, added to an agar medium in sufficient concentration, would effectively neutralize quaternary ammonium compound. In further testing, Brummer combined lecithin and polysorbate 80 in an agar medium and reported it adequately neutralized disinfectants.
Composition*
Ingredients
| g/L
|
Casein Enzymic Hydrolysate (Tryptone)
| 15.0
|
Papaic Digest of Soyabean Meal (Soya Peptone)
| 5.0
|
Sodium Chloride
| 5.0
|
Polysorbate 80 (Tween 80)
| 5.0
|
Lecithin
| 0.7
|
Histidine
| 0.5
|
Sodium Thiosulpahte
| 0.5
|
Agar
| 15.0
|
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 7.3 ± 0.2
