MacConkey Agar without CV, NaCl and with 0.15% Bile Salts Plate is used for selective isolation and differentiation of lactose fermenting and non-lactose fermenting enteric bacteria.
MacConkey Agar is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of specimens like water, faeces and other sources suspected of containing these microorganisms. The original MacConkey Agar was based on the bile salt neutral red lactose agar of MacConkey, which was used to differentiate strains of Salmonella typhosa from members of the coliform group.
Composition*
Ingredients
| g/L
|
Peptone
| 20.0
|
Lactose
| 10.0
|
Bile Salts
| 1.5
|
Neutral Red
| 0.075
|
Agar
| 12.0
|
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 7.1 ± 0.2