Mannitol Salt Agar Plate (Harmonized)

205130930100

Mannitol Salt Agar Plate is used for the isolation and cultivation of Staphylococci in accordance with microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP.

Koch reported that only Staphylococci grow on agar media containing 7.5% sodium chloride. Chapman studied this phenomenon in detail and concluded that the addition of 7.5% salt to phenol red mannitol agar results in an improved medium for the isolation of plasma coagulating Staphylococci.

Composition*

Ingredients
g/L
Pancreatic Digest of Casein
5.0
Peptic Digest of Animal Tissue
5.0
Beef Extract
1.0
D-Mannitol
10.0
Sodium Chloride
75.0
Phenol Red
0.025 
Agar
15.0

*Adjusted to suit performance parameters.
  pH at 25°C ± 2°C: 7.4 ± 0.2

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