Mueller Hinton Agar Plate (Gamma-Irradiated)

Mueller Hinton Agar Plate is used for antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by Kirby-Bauer method.

Mueller Hinton Agar was originally developed for the cultivation of Neisseria. These organisms are now isolated on selective media. Since clinical laboratories were using a wide variety of procedures for determining the susceptibility of bacteria to antibiotic and chemotherapeutic agents, Bauer, Kirby and others developed a standardized procedure in which Mueller Hinton Agar was selected as the test medium.

Composition*

*Adjusted to suit performance parameters.
  pH at 25°C ± 2°C: 7.3 ± 0.1