Mitis Salivarius Agar Plate is recommended for the isolation of Streptococci, especially Streptococcus mitis, Streptococcus salivarius and Enterococcus faecalis from grossly contaminated specimens.
Mitis Salivarius Agar is prepared as described by Chapman for the isolation of Streptococci from mixed cultures showing alpha and gamma reactions on Blood Agar. This medium (with 1% potassium tellurite) is highly selective medium which enables to isolate Streptococci from highly contaminated specimens like exudates from body cavities and faeces etc., as it inhibits a wide variety of bacteria. Some authors have also used sodium azide in this medium to inhibit the growth of Gram-negative bacteria like Proteus. Beta-haemolytic Streptococci produce colonies that resemble Streptococcus mitis.
Composition*
| Ingredients | g/L |
| Casein enzymic hydrolysate | 15.0 |
| Peptic digest of animal tissue | 5.0 |
| Dextrose | 1.0 |
| Sucrose | 50.0 |
| Dipotassium phosphate | 4.0 |
| Trypan Blue | 0.075 |
| Crystal Violet | 0.0008 |
| Agar | 15.0 |
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 7.0 ± 0.2
