Pseudomonas Agar for Detection of Fluorescein Plate is used for enhancement of fluorescein production by Pseudomonas species.
Pseudomonas species may produce water-soluble pigments in culture media. This property is sometimes used as a characteristic for the taxonomic classification of different species of Pseudomonas. Most strains of P. aeruginosa produce pyocyanin (blue) or pyoverdin (Yellow) or both, as well as pyorubrin (red), pyomelanin (Brown), or various combinations of these pigments.
P. aeruginosa and other Pseudomonas isolated from humans often produce water soluble fluorescent pigments; pyoverdin is also one of these. Fluorescent pigment-producing strains fluoresce under short-wave ultraviolet light. The fluorescence of Pseudomonas is best observed at 254 nm.
Pseudomonas Agar (for fluorescein) is a modification of formulation described by King et al., This medium is recommended by USP for use in Microbial Limit Tests.
Composition*
Ingredients
| g/L
|
Pancreatic Digest of Casein
| 10.0
|
Peptic Digest of Animal Tissue
| 10.0
|
Anhydrous Dibasic Potassium Phosphate
| 1.5
|
Magnesium Sulphate
| 1.5
|
Agar
| 15.0
|
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 7.2 ± 0.2
