Pseudomonas Agar for Detection of Pyocyanin Plate is used for enhancement of pyocyanin production by Pseudomonas species.
Pseudomonas species may produce water-soluble pigments in culture media. This property is sometimes used as a characteristic for the taxonomic classification of different species of Pseudomonas. Most strains of P. aeruginosa produce pyocyanin (blue) or pyoverdin (Yellow) or both, as well as pyorubrin (red), pyomelanin (Brown), or various combinations of these pigments.
Pseudomonas Agar (for Pyocyanin) is a modification of formulation described by King et al., This medium is recommended by USP for use in Microbial Limit Tests.
Composition*
Ingredients
| g/L
|
Pancreatic Digest of Gelatin
| 20.0
|
Anhydrous Magnesium Chloride
| 1.4
|
Anhydrous Potassium Sulfate
| 10.0
|
Agar
| 15.0
|
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 7.2 ± 0.2
