Soyabean Casein Digest Agar with 0.5% Soya Lecithin and 4% Polysorbate 20 Plate is used for determining efficacy of sanitization of containers, equipment, surfaces, water miscible cosmetics etc.
Gamma Irradiated Soyabean Casein Digest Agar with 0.5% Soya Lecithin and 4% Polysorbate 20 Plate is used for the monitoring of microbial contamination in cleanrooms and isolators. The formulation of the basic medium (Soybean Casein Digest Agar) is prepared according to the recommendations of the current European, Japanese and United States Pharmacopoeia (EP, 2.6.12.; JP, 4.05 and USP, 61) and supplemented with neutralizers.
In 1948, Weber and Black reported lecithin, added to an agar medium in sufficient concentration, would effectively neutralize quaternary ammonium compound. In further testing, Brummer combined lecithin and polysorbate 20 in an agar medium and reported it adequately neutralized disinfectants. Soyabean Casein Digest Agar with 0.5% Soya Lecithin and 4% Polysorbate 20 Plate is also used in environmental air sampling procedures.
Composition*
Ingredients
| g/L
|
Pancreatic Digest of Casein
| 15.0
|
Papaic Digest of Soyabean Meal
| 5.0
|
Sodium Chloride
| 5.0
|
Polysorbate 20
| 4.0 %
|
Soya Lecithin
| 0.5 %
|
Agar
| 15.0
|
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 7.3 ± 0.2
